Review



duolink pla blocking solution  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Millipore duolink pla blocking solution
    Duolink Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duolink+pla/pm37480622-106-6-10
    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Binding Assay:

    Article Title: Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C. ..

    Article Title: Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. ..

    Article Title: Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity
    Article Snippet: .. Non-specific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. .. The primary antibodies used were; anti-Aβ mouse IgG (1:500 4G8, Biolegends, California, USA, SIG-39220), anti-VDAC1 rabbit IgG (1:1000 abcam, Cambridge, MA, USA, ab34726) Secondary PLA probes: anti-mouse MINUS affinity-purified donkey anti-mouse IgG (H + L) and anti-rabbit PLUS affinity-purified donkey anti-rabbit IgG (H + L) were diluted 1:5 in antibody diluent buffer and samples incubated for 1 h at 37 °C followed by ligation and amplification steps as described in the manufacturer’s protocol (Duolink, Sigma-Aldrich).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice.
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 ◦C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 °C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    Proximity Ligation Assay:

    Article Title: Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C. ..

    Article Title: Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. ..

    Article Title: Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity
    Article Snippet: .. Non-specific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. .. The primary antibodies used were; anti-Aβ mouse IgG (1:500 4G8, Biolegends, California, USA, SIG-39220), anti-VDAC1 rabbit IgG (1:1000 abcam, Cambridge, MA, USA, ab34726) Secondary PLA probes: anti-mouse MINUS affinity-purified donkey anti-mouse IgG (H + L) and anti-rabbit PLUS affinity-purified donkey anti-rabbit IgG (H + L) were diluted 1:5 in antibody diluent buffer and samples incubated for 1 h at 37 °C followed by ligation and amplification steps as described in the manufacturer’s protocol (Duolink, Sigma-Aldrich).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice.
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 ◦C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 °C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    Blocking Assay:

    Article Title: Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C. ..

    Article Title: Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. ..

    Article Title: Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity
    Article Snippet: .. Non-specific binding sites were blocked with Duolink PLA blocking solution (Sigma-Aldrich) for 1 h at 37 °C. .. The primary antibodies used were; anti-Aβ mouse IgG (1:500 4G8, Biolegends, California, USA, SIG-39220), anti-VDAC1 rabbit IgG (1:1000 abcam, Cambridge, MA, USA, ab34726) Secondary PLA probes: anti-mouse MINUS affinity-purified donkey anti-mouse IgG (H + L) and anti-rabbit PLUS affinity-purified donkey anti-rabbit IgG (H + L) were diluted 1:5 in antibody diluent buffer and samples incubated for 1 h at 37 °C followed by ligation and amplification steps as described in the manufacturer’s protocol (Duolink, Sigma-Aldrich).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice.
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 ◦C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    Article Title: The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice
    Article Snippet: .. Nonspecific binding sites were blocked with Duolink PLA blocking solution (Sigma–Aldrich) for 1 h at 37 °C. .. The primary antibodies used were: anti-TLR4 rabbit IgG (1:100, Abcam, Cat#ab13556), anti-S1PR1 mouse IgG (1:100, Santa Cruz, Cat# SC-48356), and anti-CD68 Rat IgG (1:100, Abcam, Cat#ab125212).

    other:

    Article Title: Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes
    Article Snippet: Raw data were processed using Proteome Discoverer (v1.4, Thermo Fisher Scientific) and submitted for SequestHT search against the Uniprot database pertinent to the origin of the sample.



    Similar Products

    blocking solution included pla kit (duolink situ probe  (Merck & Co)
    90
    Merck & Co blocking solution included pla kit (duolink situ probe
    Blocking Solution Included Pla Kit (Duolink Situ Probe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duo92002/pm37604854-260-41-52
    Average 90 stars, based on 1 article reviews
    blocking solution included pla kit (duolink situ probe - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    duolink pla blocking solution  (Millipore)
    90
    Millipore duolink pla blocking solution
    Duolink Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duolink+pla/pm37480622-106-6-10
    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    duolink pla blocking solution  (Thermo Fisher)
    90
    Thermo Fisher duolink pla blocking solution
    TaMAB2 likely accumulates in the cytoplasm in active dimeric E3 ligase complexes. This is indicated by dimerization as well as interaction with CUL3 and presence of ubiquitin. (A) Y2H protein interaction assay of TaMAB2 with AtCUL3 and cytoskeletal proteins AtKAT, AtACT11, AtTUBG1, AtTUBG2, AtTUA6, and AtTUB8 in His prototrophy and β-galactosidase assays. For each experiment six individual colonies were analyzed. Specificity of the bait construct was confirmed by co-transformation with empty prey vectors (negative control). DMS3-RDM1 interaction served as a positive control. (B) TaMAB2-green fluorescent protein (GFP) and ubiquitin co-localize to cytoplasmic complexes in transgenic Arabidopsis protoplasts as shown by <t>Duolink</t> In Situ proximity ligation assay <t>(PLA).</t> Primary antibodies against ubiquitin and GFP were combined with secondary antibodies emitting a red fluorescent signal [Texas (TX) red] when both antibodies are in close proximity. Protoplast nuclei were stained with 4′,6-diamidino-2-phenylindole and visualized under UV light ( B , top) and merged with TX red signals ( B , bottom). A minimum of 30 protoplasts emitting a PLA signal was analyzed in three independent experiments. Protoplasts of transgenic plants overexpressing TaMAB2-GFP (line 82) are shown. Scale bar = 20 µm.
    Duolink Pla Blocking Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duolink+++pla/pmc06883508-124-12-23
    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    duolink® pla blocking solution  (Millipore)
    90
    Millipore duolink® pla blocking solution
    TaMAB2 likely accumulates in the cytoplasm in active dimeric E3 ligase complexes. This is indicated by dimerization as well as interaction with CUL3 and presence of ubiquitin. (A) Y2H protein interaction assay of TaMAB2 with AtCUL3 and cytoskeletal proteins AtKAT, AtACT11, AtTUBG1, AtTUBG2, AtTUA6, and AtTUB8 in His prototrophy and β-galactosidase assays. For each experiment six individual colonies were analyzed. Specificity of the bait construct was confirmed by co-transformation with empty prey vectors (negative control). DMS3-RDM1 interaction served as a positive control. (B) TaMAB2-green fluorescent protein (GFP) and ubiquitin co-localize to cytoplasmic complexes in transgenic Arabidopsis protoplasts as shown by <t>Duolink</t> In Situ proximity ligation assay <t>(PLA).</t> Primary antibodies against ubiquitin and GFP were combined with secondary antibodies emitting a red fluorescent signal [Texas (TX) red] when both antibodies are in close proximity. Protoplast nuclei were stained with 4′,6-diamidino-2-phenylindole and visualized under UV light ( B , top) and merged with TX red signals ( B , bottom). A minimum of 30 protoplasts emitting a PLA signal was analyzed in three independent experiments. Protoplasts of transgenic plants overexpressing TaMAB2-GFP (line 82) are shown. Scale bar = 20 µm.
    Duolink® Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duolink+pla/bio_rxiv__711424-202-13-17
    Average 90 stars, based on 1 article reviews
    duolink® pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    blocking solution from the duolink pla kit  (Thermo Fisher)
    90
    Thermo Fisher blocking solution from the duolink pla kit
    TaMAB2 likely accumulates in the cytoplasm in active dimeric E3 ligase complexes. This is indicated by dimerization as well as interaction with CUL3 and presence of ubiquitin. (A) Y2H protein interaction assay of TaMAB2 with AtCUL3 and cytoskeletal proteins AtKAT, AtACT11, AtTUBG1, AtTUBG2, AtTUA6, and AtTUB8 in His prototrophy and β-galactosidase assays. For each experiment six individual colonies were analyzed. Specificity of the bait construct was confirmed by co-transformation with empty prey vectors (negative control). DMS3-RDM1 interaction served as a positive control. (B) TaMAB2-green fluorescent protein (GFP) and ubiquitin co-localize to cytoplasmic complexes in transgenic Arabidopsis protoplasts as shown by <t>Duolink</t> In Situ proximity ligation assay <t>(PLA).</t> Primary antibodies against ubiquitin and GFP were combined with secondary antibodies emitting a red fluorescent signal [Texas (TX) red] when both antibodies are in close proximity. Protoplast nuclei were stained with 4′,6-diamidino-2-phenylindole and visualized under UV light ( B , top) and merged with TX red signals ( B , bottom). A minimum of 30 protoplasts emitting a PLA signal was analyzed in three independent experiments. Protoplasts of transgenic plants overexpressing TaMAB2-GFP (line 82) are shown. Scale bar = 20 µm.
    Blocking Solution From The Duolink Pla Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duolink+pla+blocking+solution/duolink+kit/pmc05524638-311-38-41
    Average 90 stars, based on 1 article reviews
    blocking solution from the duolink pla kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TaMAB2 likely accumulates in the cytoplasm in active dimeric E3 ligase complexes. This is indicated by dimerization as well as interaction with CUL3 and presence of ubiquitin. (A) Y2H protein interaction assay of TaMAB2 with AtCUL3 and cytoskeletal proteins AtKAT, AtACT11, AtTUBG1, AtTUBG2, AtTUA6, and AtTUB8 in His prototrophy and β-galactosidase assays. For each experiment six individual colonies were analyzed. Specificity of the bait construct was confirmed by co-transformation with empty prey vectors (negative control). DMS3-RDM1 interaction served as a positive control. (B) TaMAB2-green fluorescent protein (GFP) and ubiquitin co-localize to cytoplasmic complexes in transgenic Arabidopsis protoplasts as shown by Duolink In Situ proximity ligation assay (PLA). Primary antibodies against ubiquitin and GFP were combined with secondary antibodies emitting a red fluorescent signal [Texas (TX) red] when both antibodies are in close proximity. Protoplast nuclei were stained with 4′,6-diamidino-2-phenylindole and visualized under UV light ( B , top) and merged with TX red signals ( B , bottom). A minimum of 30 protoplasts emitting a PLA signal was analyzed in three independent experiments. Protoplasts of transgenic plants overexpressing TaMAB2-GFP (line 82) are shown. Scale bar = 20 µm.

    Journal: Frontiers in Plant Science

    Article Title: The MATH-BTB Protein TaMAB2 Accumulates in Ubiquitin-Containing Foci and Interacts With the Translation Initiation Machinery in Arabidopsis

    doi: 10.3389/fpls.2019.01469

    Figure Lengend Snippet: TaMAB2 likely accumulates in the cytoplasm in active dimeric E3 ligase complexes. This is indicated by dimerization as well as interaction with CUL3 and presence of ubiquitin. (A) Y2H protein interaction assay of TaMAB2 with AtCUL3 and cytoskeletal proteins AtKAT, AtACT11, AtTUBG1, AtTUBG2, AtTUA6, and AtTUB8 in His prototrophy and β-galactosidase assays. For each experiment six individual colonies were analyzed. Specificity of the bait construct was confirmed by co-transformation with empty prey vectors (negative control). DMS3-RDM1 interaction served as a positive control. (B) TaMAB2-green fluorescent protein (GFP) and ubiquitin co-localize to cytoplasmic complexes in transgenic Arabidopsis protoplasts as shown by Duolink In Situ proximity ligation assay (PLA). Primary antibodies against ubiquitin and GFP were combined with secondary antibodies emitting a red fluorescent signal [Texas (TX) red] when both antibodies are in close proximity. Protoplast nuclei were stained with 4′,6-diamidino-2-phenylindole and visualized under UV light ( B , top) and merged with TX red signals ( B , bottom). A minimum of 30 protoplasts emitting a PLA signal was analyzed in three independent experiments. Protoplasts of transgenic plants overexpressing TaMAB2-GFP (line 82) are shown. Scale bar = 20 µm.

    Article Snippet: After rehydration in phosphate-buffered saline, protoplasts were blocked for 30 min using Duolink PLA Blocking Solution and incubated in Duolink PLA Antibody Diluent (OLINK Bioscience) containing primary antibodies.

    Techniques: Ubiquitin Proteomics, Protein Interaction Assay, Construct, Transformation Assay, Negative Control, Positive Control, Transgenic Assay, In Situ, Proximity Ligation Assay, Staining